mouse anti her2 mab  (R&D Systems)

Journal: Pathogens

Article Title: Cellular Receptor Tyrosine Kinase Signaling Plays Important Roles in SARS-CoV-2 Infection

doi: 10.3390/pathogens14040333

Figure Lengend Snippet: Figure 3. Western blot analysis of RTK activation in A549-ACE2 cells after SARS-CoV-2 infection. Cell lysates from mock infection (Mock), A/PR8 (PR8), and SARS-CoV-2 infection at different time points were analyzed by Western blotting to detect active and total forms of EGFR (A), TrkA (B), and HER2 (C). Protein bands were quantified using Sciugo software 2.0.1. The ratio of the phosphorylated form to total form was calculated and compared to the mock (set as 1.00).

Article Snippet: The following antibodies were used for immunoblotting: rabbit anti-human TrkA polyclonal antibody (Cell Signaling Technology, 2505S, Danvers, MA, USA), rabbit anti-human pTrkA monoclonal antibody (mAb) (Cell Signaling Tech, 4168S), rabbit anti-human HER2 mAb (Cell Signaling Tech, 2165S), rabbit anti-human pHER2 mAb (Cell Signaling Tech, 2243L), mouse anti-human EGFR mAb (Cell Signaling Tech, 2239S), rabbit anti-human pEGFR mAb (Cell Signaling Tech, 3777S), rabbit anti-human GAPDH mAb (Cell Signaling Tech, 2118S), anti-rabbit HRP conjugated (Bio Rad, 1706515, Hercules, CA, USA), and anti-mouse IgG HRP conjugated (R&D, HAF007).

Techniques: Western Blot, Activation Assay, Infection, Software